Hi,
I start working with IGV, but I have some doubts in how to identify a good SPN in this program.
First I download the new Soybean Genome on Phytozome (Gmax_275_v2.0.fa and Gmax_275_Wm82.a2.v1.gene.gff3 files), and then I upload my files (sample.vcf, sample.bam and sample.bam.bai) into the program. I indexed which files that program needed, so that's OK!
But my doubt is which parameters should I consider for a good SNP? For example, what I need to see on Alleles, Genotypes and Variant Attributes? See the example below.
Chr: Chr06
Position: 35170948
ID: .
Reference: C*
Alternate: T
Qual: 160
Type: SNP
Is Filtered Out: No
Alleles:
No Call: 0
Allele Num: 2
Allele Count: 4
Allele Frequency: 1
Minor Allele Fraction: 1
Genotypes:
Non Variant: 0
- No Call: 0
Hom Ref: 0
Variant: 1Het: 0
- Hom Var: 1
Variant Attributes
AF1: 1
RPB: 5.557190e-01
VDB: 1.587578e-01
Depth: 18
FQ: -54
DP4: [1, 1, 6, 8]
AC1: 2
Mapping Quality: 25
PV4: [1, 0.22, 1, 0.24]